Fig. S5.

Cytotoxic effect of TPZ in combination with transient L-HAL and compared with doxorubicin. The HCC-bearing HBx transgenic mice were treated with 0.9% saline (n = 1), doxorubicin (10 mg/kg, n = 1), and TPZ (3 mg/kg, n = 2) through tail vein injection before transient L-HAL and killed at day 1. Total bilirubin (A) and ALT (B) in serum of these mice were determined. (C, Top) Representative gross appearance of the liver harvested from each treatment group of mice (blue dashed lines: the tumors in HAL-liver lobe; green dashed lines: the tumors in non-HAL-liver lobe). Representative H&E staining results of the tumor (C, Middle) and adjacent nontumor (C, Bottom) liver tissues of the HAL-liver lobe harvested from these mice, with the percent of the necrosis as indicated. (Scale bars: 0.2 mm.)