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. 2016 Oct 3;113(42):11913–11918. doi: 10.1073/pnas.1604047113

Fig. 1.

Fig. 1.

SSG1-1, a dominant mutation, suppressed slow growth of the sah1-1 strain and extended CLS. (A) Schematic diagram of methionine metabolism. (B) Growth of various strains on solid YPD medium. Serial dilutions of cells were spotted onto the plates, which were then incubated for 2∼3 d at 25 °C, 36 °C, or 37 °C. (C) Intracellular AdoMet and AdoHcy levels. Mean ± SD (n = 3); *P < 0.05; **P < 0.01 (t test, two-tailed, parametric unequal variance). (D) CLS curve. (E) Hydrogen peroxide stress and heat-shock stress tests. Tenfold serially diluted cells were spotted onto solid medium containing 0.6 mM hydrogen peroxide (SDC plates at 25 °C) or were subjected to heat-shock treatment at 55 °C in YPD plates, which were then transferred to 25 °C and incubated for 3 d.