Table S2.

P values for chronological lifespan analysis

Figure	Strain A	Strain B	P value against B
Fig. 1D	WT	sah1-1	<0.0001
	WT	sah1-1 SSG1-1	<0.0001
	WT	SSG1-1	<0.0001
	sah1-1	sah1-1 SSG1-1	<0.0001
Fig. 2B	WT	sam1Δ	0.4655
	WT	sam2Δ	0.0612
	WT	SSG1-1 sam1Δ	<0.0001
	WT	SSG1-1 sam2Δ	<0.0001
	SSG1-1	SSG1-1 sam1Δ	<0.0001
	SSG1-1	SSG1-1 sam2Δ	<0.0001
Fig. 2D	WT	GAL1-SAM1/2	<0.0001
Fig. 2E	WT (Control)	WT (+ 1 mM AdoMet)	0.8140
	WT (Control)	WT (+ 1 mM AdoHcy)	<0.0001
Fig. 2G	sam1Δ (Control)	sam1Δ (+ 1 mM AdoMet)	0.0141
	sam1Δ (Control)	sam1Δ (+ 1 mM AdoHcy)	0.8812
Fig. 2I	WT (Met-20 mg/L)	SSG1-1 (Met-20 mg/L)	<0.0001
	WT (Met-20 mg/L)	WT (Met-0 mg/L)	0.0490
	SSG1-1 (Met-20 mg/L)	SSG1-1 (Met-0 mg/L)	<0.0001
	WT (Met-0 mg/L)	SSG1-1 (Met-0 mg/L)	0.0002
Fig. 2J	WT (Glu-2%)	SSG1-1 (Glu-2%)	<0.0001
	WT (Glu-2%)	WT (Glu-0.05%)	0.9974
	SSG1-1 (Glu-2%)	SSG1-1 (Glu-0.05%)	<0.0001
	WT (Glu-0.05%)	SSG1-1 (Glu-0.05%)	0.9764
Fig. 2K	WT (Glu-2%)	SSG1-1 (Glu-2%)	<0.0001
	WT (Glu-2%)	WT (Glu-0.5%)	<0.0001
	SSG1-1 (Glu-2%)	SSG1-1 (Glu-0.5%)	<0.0001
	WT (Glu-0.5%)	SSG1-1 (Glu-0.5%)	0.2026
Fig. 3C	WT	snf1Δ	<0.0001
	WT	SSG1-1 snf1Δ	0.0671
	SSG1-1	SSG1-1 snf1Δ	<0.0001
Fig. 4C	WT (Glu-2%)	WT (Glu-0.5%)	<0.0001
	WT (Glu-2%)	WT (Glu-0.05%)	0.0023
	WT (Glu-2%)	WT (Glu-0%)	<0.0001
	WT (Glu-0.5%)	WT (Glu-0.05%)	<0.0001
	WT (Glu-0.05%)	WT (Glu-0%)	<0.0001
Fig. 4F	sam1Δ (Glu-2%)	sam1Δ (Glu-0.5%)	<0.0001
	sam1Δ (Glu-2%)	sam1Δ (Glu-0.05%)	0.4930
	sam1Δ (Glu-2%)	sam1Δ (Glu-0%)	0.0002
	sam1Δ (Glu-0.5%)	sam1Δ (Glu-0.05%)	<0.0001
	sam1Δ (Glu-0.05%)	sam1Δ (Glu-0%)	0.0289
Fig. S2B	WT	sah1-1 sam1Δ	<0.0001
	WT	sah1-1 sam2Δ	<0.0001
	sah1-1	sah1-1 sam1Δ	0.1231
	sah1-1	sah1-1 sam2Δ	0.8611

P values were derived from a two-way ANOVA with time and strain used as independent factors.