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. 2016 Oct 5;113(42):E6437–E6446. doi: 10.1073/pnas.1603321113

Fig. S5.

Fig. S5.

SEB acts specifically to enhance the interaction between CD28 and B7-2. (A) Low concentrations of SEB stimulate binding of B7-2 to CD28-transfected cells. HEK 293T cells were transfected to express cell-surface CD28 or with empty vector (EV). Cells were incubated with soluble B7-2 in the absence or presence of the indicated concentrations of SEB. Western blots show binding of B7-2 and expression of CD28 by the cells. Bound B7-2 is quantitated in the bar graphs (means ± SEM; n = 3). (B) Low concentrations of SEB stimulate binding of CD28 to B7-2–transfected cells. HEK 293T cells were transfected to express cell-surface B7-2 or with empty vector. Cells were incubated with soluble CD28 in the absence or presence of the indicated concentrations of SEB. Western blots show binding of CD28 and expression of B7-2 by the cells. Bound CD28 is quantitated in the bar graphs (means ± SEM; n = 3).

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