Table S1.
Kinetic parameters of surface plasmon resonance analysis with B7-2 mimetic peptides
| Fig. | Ligand | Analyte | ka, 1/ms | SEM, ka | % SEM, ka/ka | kd, 1/s | SEM, kd | % SEM, kd/kd | KD, μM | χ2 |
| 5A | pB2-4 | SEB | 413 | 10.30 | 2.50 | 9.58E−04 | 4.59E−05 | 4.80 | 2.32 | 1.05 |
| 5B | pB2-6 | SEB | 491 | 8.79 | 1.80 | 1.9E−03 | 3.69E−05 | 1.94 | 3.90 | 1.07 |
| S4A | pB2-4 | TSST-1 | 4,080 | 159.00 | 3.90 | 2.77E−03 | 8.00E−05 | 2.89 | 9.80 | 0.24 |
| S4B | pB2-6 | TSST-1 | 2,530 | 95.40 | 3.77 | 3.04E−03 | 5.92E−05 | 1.95 | 1.20 | 0.20 |
| S4C | pB2-4 | SMEZ | 3,020 | 85.00 | 2.81 | 2.93E−03 | 9.32E−05 | 3.18 | 7.30 | 0.40 |
| S4D | pB2-6 | SMEZ | 1,590 | 50.60 | 3.18 | 2.86E−03 | 1.34E−04 | 4.69 | 1.79 | 0.12 |
Purified recombinant superantigens were used. Concentrations of SEB ranged from 0.78 μM in six twofold increments; concentrations of TSST-1 ranged from 0.0625 μM in five twofold increments; and concentrations of SMEZ ranged from 0.0625 μM in four twofold increments. ka, association rate; kd, dissociation rate. BIAevaluation 3.1 software (BIAcore) was used to determine KD in the linear ligand concentration range (1:1 Langmuir binding). Criteria for KD determination for complexes were SE as percent of ka and kd; χ2 values well below 2 show the quality of fit between calculated and observed binding values and attest to the purity of the ligands examined. Fitting residuals were within the range of ±2, validating the goodness of fit.