Table S1.
Gene name | Fold change in 3D MDA at 72 h (relative to MDA control) | Fold change in 3D MDA sorted from MDA/MSC cocultures (relative to MDA control) |
Cytokines/chemokines (inflammatory response) | ||
CSF3 (GCSF) | 1.6 | 384 |
CXCL8 (IL8) | 7.1 | 1,466 |
CXCL2 | 1.8 | 107 |
PTGS2 (COX2) | 3.7 | 207 |
IL6 | −1.7 | 31 |
CXCL1 | 3.8 | 191 |
IL1A | 1.1 | 52 |
CCL20 | 2.5 | 110 |
IL1B | 1.4 | 25 |
Cell survival (drug/stress resistance) | ||
IFI6 | 2.2 | 786 |
SAT1 | 2.9 | 85 |
IER3 | −1.4 | 7.6 |
Cell growth (anticancer/senescence) | ||
EGR1 | −1.4 | 28 |
ZFP36 (TTP) | −2 | 17 |
SERPINE1 | −6.2 | 26 |
SPINT1 | 1.6 | 25 |
TFPI2 | −1.2 | 12 |
Transcription factors (stress response) | ||
FOSB | −2.1 | 19 |
JUNB | −1.2 | 15 |
JUN | −2.3 | 7.1 |
NAMPT | 3.7 | 41 |
NFKBIZ | 4.2 | 42 |
NR4A2 | 1.4 | 14 |
CEBPB | 1.2 | 12 |
Other | ||
IFI27 | 1.2 | 31 |
SLC6A14 | 1.1 | 26 |
HIST2H2BE | −2.3 | 9.4 |
TNFAIP3 | 1.7 | 31 |
MX1 | 1.2 | 25 |
IFI44L | 2.5 | 36 |
CD74 | 9.5 | 143 |
CCNL1 | 1.9 | 35 |
LAMC2 | 1.1 | 16 |
OAS2 | 1.9 | 23 |
TGFβI | −1.8 | 5.6 |
The results are compared with MDA cells cultured as monolayers (MDA control). Most genes in the list were up-regulated at least 10-fold in MDA cells from cocultures relative to 3D MDA cells. Of the genes with highest up-regulation, many are involved in inflammatory and stress responses, promote cell survival, and/or have tumor-suppressive activities.