Fig. 2.
NICD1 synergizes with multiple oncogenic pathways to drive prostate cancer. (A, Left) Schematic representation of the in vivo mouse tissue regeneration assay. Lentiviral transduction was used to overexpress GFP alone (GFP), human NICD1 and GFP (NICD1), mutant kRasG12D and RFP (kRasG12D), myristoylated/activated AKT and RFP (myrAKT), or Myc and RFP (Myc), alone or in combination with NICD1 (NICD1/kRasG12D, NICD1/myrAKT, NICD1/Myc). (Right) Transduced epithelial cells were combined with UGSM and implanted under the kidney capsule of SCID mice. Twelve weeks later the grafts were evaluated histologically. (B) Representatives of the grafts recovered 12 wk after implantation are shown for each condition. (Scale bar: 5 mm.) One of the five experiments performed is shown. (C) The recovered grafts from each condition were stained with H&E and were evaluated histologically. Representatives of the recovered grafts are shown. (Scale bars: 500 microns in upper panels; 100 microns in lower panels.)