Fig. S3.

NICD1 is highly expressed in metastatic prostate cancer. Western blot analyses were performed on metastatic CRPC tissues obtained from the rapid autopsy program at the University of Michigan using anti-cleaved Notch1 V1744 antibody (Cell Signaling Technology). GAPDH was used as a loading control. Distinct patients with metastatic disease are shown in different colors. Different colors and metastatic sites match the patients’ samples analyzed in Fig. 1C and Fig. S2.