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. 2016 Sep 30;113(42):E6457–E6466. doi: 10.1073/pnas.1614529113

Fig. S8.

Fig. S8.

NICD1 in combination with alternative pathways exhibits high self-renewal activity and metastatic potential in vivo. (A) Dissociated single cells from a primary NICD1/Myc tumor were subjected to FACS. GFP/RFP double-positive cells were sorted. GFP/RFP cells were implanted into SCID recipient mice at limiting dilutions of 10, 100, 1,000, or 10,000. (Scale bars: 0.5 cm.) (B, Left) Representative tumors initiated from 10, 100, or 1,000 cells recovered 4 wk after implantation or from 10,000 cells recovered 3 wk after implantation. (Scale bars: 1 cm.) (Right) H&E staining of each tumor is shown. (Scale bars: 50 microns.) One of the two independent experiments is shown. (C) The Myc-CAP cell line (44) has lower self-renewal activity and was used as a control. Representative tumors initiated from 10, 100, or 1,000 Myc-CAP cells recovered 8 wk after implantation. (Scale bar: 1 cm.)