. 2016 Jul 2;62(5):521–526. doi: 10.1262/jrd.2016-057

Table 1. Developmental ability of Wistar oocytes matured in vitro in various culture media.

Culture media	No. of oocytes collected from ovaries	No. (%) of oocytes matured to MII	No. of MII oocytes activated	No. (%) of oocytes after activation
Culture media	No. of oocytes collected from ovaries	No. (%) of oocytes matured to MII	No. of MII oocytes activated	Pronuclear stage	MII stage (non-activated)	Degenerated and fragmented
In vivo	-	-	32	29 (91) ^c	3 (9)	0 (0)
HTF	109	100 (92) ^a	100	34 (34) ^d	50 (50)	16 (16)
αMEM	137	110 (80) ^b	110	86 (78) ^e	17 (15)	7 (6)
HTF + αMEM	183	169 (92) ^a	169	92 (54) ^f	63 (37)	14 (8)
TYH + αMEM	168	142 (85)	142	107 (75) ^g	27 (19)	8 (6)

The percentages of oocytes that matured to MII and those that formed a pronucleus after activation were statistically analyzed. P < 0.05: a vs. b; c vs. d and f; d vs. e, f, and g; e vs. f; f vs. g (chi-square test followed by multiple comparison using Ryan’s method).