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. Author manuscript; available in PMC: 2016 Oct 27.
Published in final edited form as: Curr Opin Chem Biol. 2015 Aug 4;28:123–130. doi: 10.1016/j.cbpa.2015.07.007

Figure 1.

Figure 1

Complementary strategies for generating fluorescently labeled proteins using Uaa mutagenesis that rely on genetic encoding of the protein of interest with a nonsense codon and an orthogonal tRNA/synthetase pair to incorporate the Uaa cotranslationally. Top: Incorporation of a fluorescent Uaa leads directly to fluorescently labeled protein. Bottom: A complementary strategy based on Uaa incorporation and subsequent functionalization allows for modular fluorophore incorporation.