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. Author manuscript; available in PMC: 2016 Oct 27.
Published in final edited form as: Curr Opin Chem Biol. 2015 Aug 4;28:123–130. doi: 10.1016/j.cbpa.2015.07.007

Figure 2.

Figure 2

Double-labeled proteins produced through Uaa mutagenesis and expressed protein ligation. (A) Incorporation of N-terminal thioamides through semisynthesis. (B) Incorporation of C-terminal thioamides through expressed protein ligation, where the expressed fragment is fused to a C-terminal intein. (C) Measurements of protein compaction in the presence of trimethylamine-N-oxide (TMAO) using α-synuclein containing a cyanophenylalanine (F*) and a thioamide (Thio-Ala, As or Thio-Phe, Fs) FRET pair; subscript indicates position in the α-synuclein sequence.