Table 5. Current practice in assessment of anti-dsDNA testing.
| Anti-dsDNA testing (N = 15) |
N (proportion) |
|---|---|
| Methods | |
| FEIA | 6 (0.4) |
| ELISA | 5 (0.33) |
| MIA (Luminex) | 3 (0.2) |
| CLIFT | 1 (0.07) |
| Results reporting | |
| quantitative | 14 (0.93) |
| qualitative | 1 (0.07)* |
| Units reported | |
| IU/mL | 7 (0.47) |
| kIU/L | 2 (0.13) |
| U/mL | 3 (0.2) |
| AU/mL | 2 (0.13) |
|
Algorithm applied for anti-dsDNA testing in regards to ANA-screen test (N = 12) ** |
N (proportion) |
| Only when requested, regardless of the ANA-screen result | 6 (0.5) |
| As a reflex test on ANA-screen positive result | 4 (0.33) |
| Only when requested and ANA-screen result is positive | 1 (0.08) |
| Only when requested and ANA titer > 1 / 160 | 1 (0.08) |
| *Laboratory that uses CLIFT method. **Answers obtained for 12/15 laboratories. FEIA = Fluorescence enzyme immunoassay. ELISA = Enzyme-linked immunosorbent assay. MIA = Multiplex bead immunoassay. CLIFT = Crithidia luciliae immunofluorescence test. | |