Table 7. Current practice in immunodiagnostic of celiac disease.
| Antibodies used in immunodiagnostic of celiac disease (N = 11) |
N (proportion) |
|---|---|
| Methods used for anti-tTG determination (N = 11) | |
| ELISA | 3 (0.27) |
| FEIA | 5 (0.45) |
| MIA (Luminex) | 2 (0.18) |
| Immunocromatography | 1 (0.09) |
| Methods used for anti-DPG determination (N = 5) | |
| FEIA | 3 (0.6) |
| MIA (Luminex) | 2 (0.4) |
| Methods used for EMA determination (N = 4) | |
| IIF | 3 (0.75) |
| ELISA | 1 (0.25) |
| Methods used for AGA determination (N = 3) | |
| MIA (Luminex) | 2 (0.67) |
| FEIA | 1 (0.33) |
|
IgG class of antibodies associated with celiac disease (N = 5) | |
| Anti-tTG-IgG | 2 (0.4) |
| Anti-DGP-IgG | 3 (0.6) |
| Algorithms in diagnosis of celiac disease (N = 11) | |
| Measurement of total IgA precedes determination of IgA class antibodies | 5 (0.45) |
| IgG class of antibodies associated with celiac disease determined only in the cases of IgA deficiency and in children ≤ 2 years old. | 3 (0.27) |
| IgG class of antibodies associated with celiac disease always determined together with IgA class antibodies. | 2 (0.18) |
| anti-tTG = antibodies against tissue transglutaminase. ELISA = Enzyme-linked immunosorbent assay. FEIA = Fluorescence enzyme immunoassay. MIA = Multiplex bead immunoassay. anti-DGP = antibodies targeting deamidated gliadin peptides. EMA = anti-endomysium antibodies. IIF = Indirect immunofluorescence. AGA = antibodies against native gliadin. | |