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. 2016 Oct 27;7:1546. doi: 10.3389/fpls.2016.01546

Figure 3.

Figure 3

Effects of acyA disruption on E. festucae hyphal morphology in axenic culture. Bright field images of E. festucae Fl1 hyphae growing on water agar at 22°C for 7 days (bar, 10 μM). Images were taken approximately 3 mm behind the colony margin. Hyphae of all independent mutants (ΔacyA34, ΔacyA42, and ΔacyA47) were convoluted, irregular in diameter (ΔacyA47) and heavily branched compared to the wild-type. Ectopic integration of the wild-type acyA gene into ΔacyA34 and ΔacyA42 resulted in a reversion to the wild-type phenotype to greater or lesser extents dependent on the individual strain (ΔacyA34/acyA, ΔacyA42/acyA). Gross morphology at the hyphal tips appeared similar in mutants and wild-type.