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. 2016 Oct 27;7:1546. doi: 10.3389/fpls.2016.01546

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Copyright © 2016 Voisey, Christensen, Johnson, Forester, Gagic, Bryan, Simpson, Fleetwood, Card, Koolaard, Maclean and Johnson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology of E. festucae ΔacyA in L. perenne. Aniline blue-stained hyphae (arrows) in the mature second leaf sheath of L. perenne infected with the wild-type and strains ΔacyA34 and ΔacyA42. The morphology of the mutant strains after complementation (ΔacyA34/acyA and ΔacyA42/acyA) with the wild-type gene is also shown. All images are at the same magnification. The scale bar is 5 μm.