Skip to main content
. Author manuscript; available in PMC: 2016 Oct 27.
Published in final edited form as: Nat Methods. 2015 Apr 6;12(5):439–444. doi: 10.1038/nmeth.3357

Figure 4. Escape-incompetent Listeria monocytogenes, deficient in listeriolysin O and broad range phospholipase C, nucleate actin after BLAST delivery directly into the cytosol of NHDFs.

Figure 4

L. monocytogenes-GFP Δhly ΔplcB (green), strain 10403S, was delivered by BLAST with laser pulsing into the cytosol of NHDFs (a) or, as a sham control, in the absence of laser pulsing (b, c). Nuclei, stained with DAPI, fluoresce blue and F-Actin, stained by phalloidin-rhodamine, fluoresces red. A higher magnification of a green fluorescent bacterium and its actin comet tail after BLAST delivery with laser pulsing is shown (a, inset). In the absence of laser pulsing, intracellular bacteria are rare and comet tails are not seen (b, c, inset). Scale bars, 20 µm and 5 µm for zoom-in images. The experiment was conducted twice with the same results each time.