Figure 4. Example of nested PCR with primers R16F2n/R2 for the detection of phytoplasma in the sucrose media fed upon by field-collected insects.

Numbers 1–3 represent unidentified Zoraidini species; Numbers 4–8 represent Lophops saccharicida (nymph); Numbers 9–14 represent Colgar sp.; Numbers 15–17 represent Zophiuma pupillata; M = DL 2000 marker; (+) = positive control; and (−) = negative control.