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. 2016 Jul 4;20(11):2122–2137. doi: 10.1111/jcmm.12909

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) The number of RGCs was evaluated via anti‐Brn‐3a staining (green) at 14 days. Cell nuclei were labelled with DAPI (blue). (B) The RGC count showed no differences between both immunized groups compared with the Co group (ONA: P = 0.3; S100: P = 0.4). (C) The mRNA expression of the RGC marker Pou4f1 revealed no changes in either group (ONA: P = 0.4; S100: P = 0.6). Values for immunostaining are mean ± S.E.M. Values for qRT‐PCR are median ± quartile ± maximum/minimum. GCL: ganglion cell layer; scale bar: 20 μm.