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. 2016 Jul 4;20(11):2122–2137. doi: 10.1111/jcmm.12909

Figure 6.

Figure 6

(A) Staining of retinal cross‐sections with anti‐GFAP (green), anti‐vimentin (red), anti‐glutamine synthetase (green) and DAPI (cell nuclei, blue) 14 days after immunzation. In the control group GFAP is mostly expressed in the nerve fibre and ganglion cell layer (arrow). Vimentin and glutamine synthetase seem to label mainly Müller cells in the retina (arrows). (B) The GFAP + area analysis revealed no alterations in both immunized groups (P > 0.05). No changes in vimentin and glutamine synthetase staining could be observed for the ONA and the S100 group at 14 days (P > 0.05). (C) The qRT‐PCR revealed an increase in GFAP expression in the S100 group (p < 0.001), whereas the ONA group was not affected (P > 0.05). (D) For vimentin expression, no alterations were observed in either groups (P > 0.05). (E) Also, the expression of glutamine synthetase was not altered in the ONA and S100 group (P > 0.05). Values for immunostaining are mean ± S.E.M. and for qRT‐PCR median ± quartile ± maximum/minimum. GCL: ganglion cell layer; IPL: inner plexiform layer; GS: glutamine synthetase; scale bar: 20 μm; *** P < 0.001.