Table 2.
Adjustments set for ImageJ macro. Before using the macro for area analysis of tenascin‐C, 473HD, GFAP, vimentin and glutamine synthetase, the background subtraction as well as the lower and the upper threshold must be set
| Protein/epitope | Tissue | Background subtraction (pixel) | Lower threshold | Upper threshold |
|---|---|---|---|---|
| GFAP | Retina | 100 | 19.49 | 201 |
| Glutamine synthetase | Retina | 20 | 7.95 | 80.5 |
| Phosphacan = 473HD epitope | Retina | 0 | 24.29 | 85 |
| Optic nerve | 50 | 13.77 | 85 | |
| Tenascin‐C | Retina | 0 | 28.24 | 85 |
| Optic nerve | 50 | 13.03 | 85 | |
| Vimentin | Retina | 100 | 20.16 | 247 |