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. 2016 Jul 11;20(11):2173–2182. doi: 10.1111/jcmm.12917

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© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Analyses of adipogenesis and osteogenesis in the femoral head. (A) Haematoxylin–eosin staining for observation of empty lacunae (black arrow), thickness and density of trabeculae, bone marrow cell necrosis (*), and numbers and sizes of adipocytes in the femoral head of rats that received saline (control treatment), GFP‐BMSCs or MDR1‐GFP‐BMSCs. (B) Rate of empty lacunae. (C) Adipose tissue area. (D) Adipocyte number. (E) Adipocyte perimeter. (F) Immunohistochemical staining for GFP in sections of the femoral head. Positive staining mainly occurred in cells (black arrowhead) attached to the surface of the trabeculae and shaped like osteoblasts after transplantation of GFP‐BMSCs or MDR1‐GFP‐BMSCs. (G) Tetracycline fluorescence indicating new bone formation. (H) MAR. In all panels, data are expressed as mean ± S.D. ***P < 0.01 relative to control group and GFP‐BMSC transplant group. ^### P < 0.01 relative to control group.