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. 2016 Jul 15;20(11):2194–2207. doi: 10.1111/jcmm.12926

Figure 4.

Figure 4

The reduction of ER Ca2+ levels induced by expression of R321X is not caused by changes in [Ca2+]cyt. (A) The steady‐state D1ER FRET signal (ratio 530/470 nm) of HEK293 cells expressing either mCh‐Lamin A or mCh‐R321X is depicted in pseudocolor. The histogram compares changes in netFRET (as % of control) among mock cells (n = 64 cells) and cells expressing either mCh‐Lamin A (n = 74 cells) or mCh‐R321X (n = 72 cells). Data are expressed as means ± S.E.. Statistical analysis was performed on three independent experiments and significance calculated by anova. ***P < 0.001 versus MOCK, °°°P<0.001 versus Lamin A. (B) HEK293 cells expressing either mCh‐Lamin A (black bar) or mCh‐R321X (grey bar) were loaded with Fura‐2 and the free cytosolic [Ca2+]cyt was calculated as described in the Methods. Data are expressed as mean ± S.E.. Statistical analysis was performed on three independent experiments and significance calculated by Student's t‐test for unpaired samples. P = n.s.