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. 2016 Jul 15;20(11):2194–2207. doi: 10.1111/jcmm.12926

Figure 8.

Figure 8

LMNA R321X expression by itself increases the rate of cell apoptosis. (A) Detection of apoptosis by flow cytometry. Expression of GFP‐R321X increased the % of apoptotic cells when compared with cells expressing GFP‐Lamin A. Data are expressed as mean ± S.E.. Statistical analysis was performed on three independent experiments and significance calculated by Student's t‐test for unpaired samples. ***P < 0.001. PerCP‐CY5.5‐A, peridinin‐chlorophyll proteins conjugate with CY5.5, FITC, fluorescein isothiocyanate, Q1, GFP positive cells; Q2, GFP positive apoptotic cells; Q3, GFP negative cells; Q4, GFP negative apoptotic cells. (B) Lysates from HEK293 cells expressing either GFP‐Lamin A or GFP‐R321X were subjected to Western blot analysis to detect the expression of either full length‐ or cleaved PARP (PARP FL and PARP CL respectively). The statistical analysis graph of relative expression levels of PARP FL and PARP CL normalized to that of β‐actin. Each experiment was repeated in triplicate. Data are expressed as mean ± S.E.. Statistical analysis was performed on three independent experiments and significance calculated by Student's t‐test for unpaired samples. ***P < 0.001 versus GFP‐Lamin A –PARP FL.