Table 1.

selected milestones in the developing of protein based CEST agents

	Authors	Year	Topic	Findings relevant to the next gen LRP	Ref.
1	McMahon et. al.	2008	Quantifying exchange rate	Determining the kex of exchangeable protons	(70)
2	Gilad et. al.	2007	LRP prototype	LRP can be expressed in the brain	(77)
3	McMahon et. al.	2008	Multicolor peptides	Changing the amino acid sequence affect the resonances frequency and the contrast intensity	(82)
4	Liu et. al.	2010	Peptide screening with MRI	Developing tools for high-throughput screening	(69)
5	Airan et. al.	2012	PKA sensor	(1) Synthetic gene that with heterogeneous amino acid sequence (2) effect of phosphorylation on the contrast	(72)
6	Bar-Shir et. al.	2014	Human protamine as a reporter gene	DNA optimization improves contrast	(75)
7	Oskolkov et. al.	2014	Characterization of Human Protamine	(1) Effect of phosphorylation and (2) effect of intermolecular bonds on the contrast	(76)
8	Bar-Shir et. al.	2015	Supercharged GFP as a CEST based reporter	The contrast can be improved by changing only limited number of amino acids	(74)