Skip to main content
. 2016 Aug 8;102(2):290–305. doi: 10.1111/mmi.13460

Figure 3.

Figure 3

Rss1 localizes to U. maydis nuclei and responds to SA as a transcriptional activator in a heterologous system.

A. mCherryHA‐Rss1 localizes to the nuclei of U. maydis yeast‐like cells. Localization of mCherryHA‐Rss1 in cells of axenically grown CL13Δrss1‐mCherryHA‐rss1 culture was assessed by confocal laser scanning microscopy. Cells were stained with DAPI to visualize nuclei. Scale bars: 10 μm.

B. Gal4‐BD‐Rss1 activates reporter gene expression in S. cerevisiae in the presence of salicylate and shows a dose‐dependent response to SA. AH109 expressing Gal4‐BD (AH109‐BD; negative control), Gal4‐BD‐rss11‐216 (AH109‐BD‐Rss11‐216; positive control), or Gal4‐BD‐rss1 (AH109‐BD‐Rss1) was spotted in serial dilutions on SD‐Trp, SD‐Trp‐Ade‐His, SD‐Trp‐Ade‐His + 100 µM sodium salicylate and SD‐Trp‐Ade‐His + 1 mM sodium salicylate. Under stringent conditions growth of AH109‐BD‐Rss1 was only detectable with addition of salicylate.