Skip to main content
. 2016 Aug 8;102(2):290–305. doi: 10.1111/mmi.13460

Figure 7.

Figure 7

Anthranilate can induce Rss1 activity in yeast. The yeast‐based transcriptional activation assay was performed with anthranilate as a putative inducer. AH109 expressing Gal4‐BD (AH109‐BD; negative control), Gal4‐BD‐rss11‐216 (AH109‐BD‐Rss11‐216; positive control), or Gal4‐BD‐rss1 (AH109‐BD‐Rss1) were spotted in serial dilutions on SD‐Trp (growth control) (A), on SD‐Trp‐Ade‐His (B) and on SD‐Trp‐Ade‐His + 1 mM anthranilic acid (C). Addition of anthranilic acid resulted in an activation of reporter gene expression and growth of AH109‐BD‐Rss1.