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. 2016 Oct 26;22:1267–1279.

Table 1. Primer list of CHST6 and TGFBI genes.

CHST6 Sequences of primers (5′-3′) Tm(°C) PCR product
Primer 1
F: CTCGGGTCTGGTGGTAGAATCT
R: TTGAAGAAGCGCACCTCCTTG
60.9
61.1
658 bp
Primer 2
F: CTCTTCCAGTGGGCCGTGAG
R: TTGAGCGCATTCCTGGACGAA
62,5
62,6
626 bp
Primer 3
F: GCAGAAATCCGTGCGCTCTAC
R: AGAGAAAGAAACGTGCAGTCCTT
61,9
60,4
505 bp
TGFBI
Sequences of primers (5′-3′)
Tm(°C)
PCR product
Exon 4
F: TCGTCCTCTCCACCTGTAGA
R: AACATGTTCTCAGCCCTCGT
59,0
59,3
548 bp
Exon 12 F: AACCAAGGTGTGTGCATTCC
R: TTTAGTCCCGCCCACTCTTT 59,0
59,0 415 bp