Table 2. Excessive axial length in the KO originates solely from an increased vitreous chamber depth.
| Dimension | Mean | SD | Sample number | Mean | SD | Sample number | ANOVA p-value Tukey | Significant at p<0.05? |
|---|---|---|---|---|---|---|---|---|
| Wild type P30 |
Knockout P30 |
|||||||
| CCT |
100.1 |
10.58 |
8 |
109.4 |
9.18 |
6 |
ns |
No |
| ACD |
289.6 |
12.49 |
8 |
259.4 |
14.14 |
6 |
ns |
No |
| Lens |
1800.9 |
41.2 |
8 |
1787 |
29.19 |
6 |
ns |
No |
| Vitreous |
638.4 |
37.59 |
8 |
1018.4 |
51.26 |
6 |
< 0.0001 |
Yes |
| Retina |
210.5 |
24.94 |
8 |
172 |
16.39 |
6 |
< 0.05 |
Yes |
| Total axial length |
3067.6 |
56.52 |
8 |
3353.9 |
35.44 |
6 |
< 0.0001 |
Yes |
| Wild type P45 |
Knockout P45 |
|||||||
| CCT |
87.9 |
12.13 |
7 |
90.27 |
9.07 |
8 |
ns |
No |
| ACD |
318.1 |
15.13 |
7 |
296.04 |
15.99 |
8 |
ns |
No |
| Lens |
1918.6 |
38 |
7 |
1920.62 |
30.86 |
8 |
ns |
No |
| Vitreous |
653.7 |
15.77 |
7 |
971.27 |
52.03 |
8 |
< 0.0001 |
Yes |
| Retina |
208.9 |
15.77 |
7 |
183.37 |
15.48 |
8 |
ns |
No |
| Total axial length |
3224 |
55.22 |
7 |
3475.17 |
27.58 |
8 |
< 0.0001 |
Yes |
| Wild type P53-55 |
Knockout P53-55 |
|||||||
| CCT |
111 |
4.4 |
3 |
112.6 |
5.397 |
5 |
ns |
No |
| ACD |
314.5 |
4.49 |
3 |
294.7 |
10.92 |
5 |
ns |
No |
| Lens |
1866 |
14.93 |
3 |
1849 |
16.74 |
5 |
ns |
No |
| Vitreous |
564 |
8.73 |
3 |
899.6 |
15.12 |
5 |
< 0.0001 |
Yes |
| Retina |
274.1 |
12.7 |
3 |
239.4 |
27.7 |
5 |
ns |
No |
| Total axial length |
3130 |
9.91 |
3 |
3396 |
36.34 |
5 |
< 0.0001 |
Yes |
| Wild type P80-82 |
Knockout P80-82 |
|||||||
| CCT |
87.3 |
7.44 |
5 |
95.5 |
7.61 |
7 |
ns |
No |
| ACD |
365.2 |
26.35 |
5 |
354.7 |
33.19 |
7 |
ns |
No |
| Lens |
2099 |
81.37 |
5 |
2075.5 |
32.13 |
7 |
ns |
No |
| Vitreous |
530.3 |
60.93 |
5 |
883.8 |
18.17 |
7 |
< 0.0001 |
Yes |
| Retina |
222 |
13.84 |
5 |
175 |
19.59 |
7 |
< 0.05 |
Yes |
| Total axial length | 3359.9 | 34.21 | 5 | 3615 | 63.63 | 7 | < 0.0001 | Yes |
Axial measurements were made with a Bioptigen 4310. Axial measurements were collected in the KO versus WT mouse eyes at P30, P45, P54, and P80. All measurements are in microns. ANOVA and a Tukey test were conducted. The essential point of these axial measurements is that the vitreous chamber of the KO was much deeper than the WT, and this accounted for all the excess axial length of the KO mouse. Abbreviations: CCT, central corneal thickness; ACD, anterior chamber depth; Lens, lens thickness; Vitreous, Vitreous Chamber depth; Retina, retina thickness; Length, total axial length from anterior surface of the cornea to the basal face of the RPE.