Figure 2. Xvelo forms a stable matrix.
(A) mRNA encoding for Xvelo-GFP was microinjected into stage I oocytes. Mitotracker Deep Red was used to label mitochondria. Oocytes were imaged live with a scanning confocal microscope with a 40× Water immersion objective.
(B) Magnification of the Balbiani body in (A).
(C) Internal rearrangement of fluorescent Xvelo-GFP particles after half bleach over time.
(D) The fluorescent recovery of the half-bleached Xvelo-GFP in the Balbiani body in (C) and two other biological replicates are shown by quantification of fluorescence in bleached region over time. Fluorescent intensity changes in the bleached region per pixel over time were plotted after it was normalized for photobleaching by using an unbleached neighbouring area and background subtraction.
See also Figure S2