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Dynamics of TYLCV and autophagy in the gut of the whitefly. Whiteflies were infected with TYLCV for 6 h on tomato and then transferred onto cotton for 0 to 288 h. Guts of viruliferous whiteflies were dissected, fixed and labeled with anti-CP (A) and anti-ATG8 (B) antibodies. Bar: 20 μm. Blue indicates DAPI staining of the nuclei. For each time point, 20 midguts were dissected and similar trend was observed. (C) Localization of CP and ATG8 on the same sample.
