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. 2016 Jun 16;12(9):1560–1574. doi: 10.1080/15548627.2016.1192749

Figure 4.

Figure 4.

Effects of inhibiting autophagy on TYLCV. (A) TYLCV CP and ATG8 of whiteflies treated with 3-MA (+) or without 3-MA (−) were detected by western blot. (B) TYLCV DNA was detected by qPCR (Significant differences are indicated with different letters, P < 0.05). (Cto E) TYLCV gene expression (V1, V2 and C3) were also detected by qRT-PCR (Significant differences are indicated with different letters, P < 0.05). (F) TYLCV CP, ATG8 and SQSTM1/p62 in whiteflies treated with (+) and without BAF (−) were detected by western blot. (G) The effect of BAF treatment on the accumulation of TYLCV CP at 48 h was detected by immunofluorescence. (H) The effect of BAF treatment on the accumulation of the TYLCV genome. TYLCV DNA was detected by qPCR at different time points (significant differences are indicated with different letters, P < 0.05). (I) Silencing of whitefly Atg3 and Atg9 by feeding double-strand RNA (dsRNA). The expression of Atg3 and Atg9 genes was monitored by qRT-PCR (significant differences are indicated with different letters, P < 0.05). (J) The level of ATG8, SQSTM1/p62 and TYLCV CP was then analyzed by immunoblot. (K) The effect of silencing Atg3 and Atg9 on the accumulation of the TYLCV genome. TYLCV DNA was detected by qPCR (Significant differences are indicated with different letters, P < 0.05).