Figure 6.

Hypoxic induction of BNIP3 does not correlate with the activation status of BECN1. (A) Western blot analysis of NP cells cultured under normoxia (NX) or hypoxia (HX) for 8–72 h shows that the BNIP3 level significantly increases by 24 h in hypoxia. BNIP3L level as well as p-BECN1 Ser93 did not change under hypoxia. (B) Densitometric analysis of western blot confirms hypoxic induction of BNIP3, peaking at 24 h. In contrast, the BNIP3L level remains relatively constant in hypoxia except for a small decrease at 72 h. Additionally, the level of p-BECN1 Ser93 is not affected under hypoxia, indicating a lack of correlation between BNIP3 and activation status of BECN1. All the quantitative data are represented as mean ± SE from at least 3 independent experiments. NS, nonsignificant; *, p < 0.05.