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. 2016 Oct 27;11(10):e0164326. doi: 10.1371/journal.pone.0164326

Fig 4. Identification of 18:0/22:5(OH)-PE by LC/MS/MS.

Fig 4

(a) Single reaction monitoring chromatogram of the 808.8 → 345.2 transition, with solvent flow reduced to 850 μl/min. 18:0/22:5(+O)-PE eluted 0.3 min later than 18:0/22:5-PE, consistent with the slightly greater polarity of the oxidized species. (b) Product ions produced by the m/z 808.8 parent ion at 12.6 min. Due to the low concentrations of the parent species (a maximum of only 400 transitions/sec observed in panel a), this spectrum was collected with the Q1 resolution set to “unit”, while the Q3 resolution was set to “open”. The position of the oxygen on the 22:5 chain is unknown, and possibly variable. The identity of the m/z 672.9 and 749.5 product ions is not known.