| Reagent | Final Concentration (mM) | MW or Concentration of stock | 500 ml |
|
| |||
| NMDG | 93 | 195.2 | 9.08 g |
| HCl | 12.1 N | 3.5 ml | |
| KCl | 2.5 | 2.5 M | 500 μl |
| NaH2PO4 | 1.25 | 1.25 M | 500 μl |
| NaHCO3 | 30 | 84.0 | 1.26 g |
| HEPES | 20 | 2 M | 5 ml |
| Glucose | 25 | 2.5 M | 5 ml |
|
| |||
| Ascorbic acid | 5 | 176.1 | 0.44 g |
| Thiourea | 2 | 76.1 | 0.08 g |
| Na+ pyruvate | 3 | 110.0 | 0.17 g |
|
| |||
| MgSO4 | 10 | 2 M | 2.5 ml |
| CaCl2 | 0.5 | 1 M | 250 μl |
|
| |||
| N-acetylcysteine | 12 | 163.2 | 0.98 g |
|
| |||
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.