Fig 2. The effect of E2 treatment on the expression of PUFA synthesis enzymes in MCF7 cells.

MCF7 cells were treated with 10 nM E2 and/or 10 μM ICI182,780 compared or vehicle (ethanol) for 6 hours. Elovl2, Elovl5, Fads2 and Fads1 mRNA levels (A, B, C and D) were determined by quantitative RT-PCR and normalized to the reference gene 36B4. Results shown are means ± SE of two individual experiments in triplicate. Statistical significances are indicated as *P<0.05 and **P<0.01.