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. 2016 Oct 27;11(10):e0164241. doi: 10.1371/journal.pone.0164241

Fig 5. ERα overexpression does not modify the expression of PUFA elongases and desaturases in MCF7 cells.

Fig 5

MCF7 cells were transfected with different concentrations (50ng or 500ng) of ERα or empty plasmid (V) as indicated for 24 hours followed by incubation with 10 nM E2 or vehicle (c) for 6 hours. (A) ERα, (B) ERβ, (C) Elovl2, (D) Elovl5, (E) Fads1 and (F) Fads2 mRNA expression were determined by quantitative RT-PCR normalized to the reference gene 36B4. Results shown are means ± SE of three individual experiments in triplicate. Statistical significances are indicated as *P<0.05, **P<0.01 and ***P<0.001.