Fig 7. ERα knock-down reduces Elovl2 expresion in MCF7 cells.

MCF7 cells were transiently transfected with 50 nM ERα siRNA or siRNA control for 48 hours followed by incubation with 10 nM E2 (black bars) or vehicle (white bars) for 4 hours. (A) Western blot analysis using total protein extracts was performed to assess ERα and β-actin (control) protein levels. (B) Quantification of ERα protein levels, and (C) Elovl2 and (D) Elovl5 mRNA expression levels determined by quantitative RT-PCR normalized to the reference gene 36B4. Results shown are means ± SE of two individual experiments in duplicate. Statistical significances are indicated as *P<0.05, **P<0.01 and ***P<0.001.