TABLE 2 .

Proteins copurified with EccC₅strep^a

Identified protein	Protein description	MW	Sequence coverage (%)	MS/MS normalized spectral count				Fold change	P value	NSAF
				WT		eccC5-strep
				A	B	A	B			A	B
EccC5	ESX-5 core component	152.6	71.4	75	50	706	651	10.8	5.7 × 10−6	0.42	0.41
EccD5	ESX-5 core component	53.5	29.2	24	15	118	111	5.9	4.4 × 10−5	0.20	0.20
EccE5	ESX-5 core component	44.0	57.6	17	8	103	110	8.5	4.3 × 10−5	0.21	0.24
EccB5	ESX-5 core component	54.1	55.4	22	16	95	84	4.6	8.3 × 10−5	0.16	0.15
MycP5	ESX-5 component	59.8	29.5	0	3	9	19	6.9	4.8 × 10−3

^aLC-MS/MS was performed on Strep-tag-purified material from M. marinum wild-type (negative control) and M. marinum-ΔeccC₅-eccC₅strep cell envelope fractions, followed by a two way analysis. Proteins that showed >10 normalized spectral counts in both eccC₅strep pulldown samples and a normalized spectral abundance factor (NSAF) of >0.05 were selected. The NSAF was calculated by dividing the normalized spectral counts from the nanoLC-MS/MS experiment by the relative molecular weight (M_r) to obtain the spectral abundance factor (SAF) for each protein. Subsequently, each SAF was normalized by dividing it by the sum of the SAFs of the proteins in the complex. Data in columns A and B represent results from biological replicates. MW, molecular weight.