Table 3. Antibody titre (IgG) obtained by IFAT and detection of Toxoplasma gondii in lambs, stillborns or foetuses from primarily infected ewes (ME49 strain) that were reinfected with 2.5 x 10³ oocysts (VEG strain) of T. gondii and ewes only primarily infected (ME49 strain).

Group	Ewe number/		T. gondii antibody titre (IFAT)/Days after birth			Detection of T. gondii (methods)
	Respective lamb		Immediately after birth (pre-colostral)	3 days	14 days
I: Reinoculation at 40 days of gestation	958	Lamb	64	64	32	(B, I and P)
	970	Lamb	64	NP	NP	(I and P)
		Foetus	64	NP	NP	(B and I)
	979	Lamb	64	64	32	(I and P)
	1039	Lamb	128	64	32	(I)
	1048	Lamb	64	64	NP	(B, I and P)
II: Reinoculation at 80 days of gestation	972	Lamb	256	64	64	(B, I and P)
	974	Lamb	64	64	32	(B, I and P)
	975	Lamb 1	-	64	NP	(B)
		Lamb 2	128	NP	NP	(B, I and P)
	980	Lamb 1	-	64	64	(B, I and P)
		Lamb 2	128	128	32	(B, I and P)
	1016	Stillborn	128	NP	NP	(B, I and P)
III: Reinoculation at 120 days of gestation	1019	Lamb	1024	NP	NP	(B, I and P)
	1027	Lamb	512	NP	NP	(B, I and P)
		Foetus	128	NP	NP	(B and I)
	1038	Lamb	1024	512	128	(B, I and P)
	1041	Lamb	128	NP	NP	(B and I)
		Stillborn	512	NP	NP	(B and I)
	1049	Lamb	512	NP	NP	(B, I and P)
		Stillborn	256	NP	NP	(I and P)
IV: negative control for reinfection	1017	Foetus 1	-	NP	NP	(B, I and P)
		Foetus 2	64	NP	NP	(B, I and P)
		Foetus 3	32	NP	NP	(B, I and P)
		Foetus 4	32	NP	NP	(B, I and P)
		Foetus 5	-	NP	NP	(B, I and P)
	1044	Lamb 1	64	NP	NP	(B, I and P)
		Stillborn	32	NP	NP	(B, I and P)
	1046	Lamb	-	≥32	≥32	(B, I and P)
	1023	Lamb	-	≥32	≥32	(B)
V: negative control for primary infection	903	-	-	-	-	-
	922	-	-	-	-	-
	956	-	-	-	-	-
	944	-	-	-	-	-
	1051	-	-	-	-	-

-: negative (IFAT < 32); B: Bioassay in mice; I: Immunohistochemistry; P: PCR

NP: not performed