Table 1. Results summary.
Phage gene expression | Host gene expression | Pyridine nucleotides | Productivity of infection | |
---|---|---|---|---|
Light early | Early genes: high at 30 min, max at 1.5 h Middle genes: high at 1.5 h, max at 2.5 h | ↑ HLIPs, PTOX, PET, Trx, PSI ↓ Cell div, Trx, Transl | Slight increase in NADPH/NADP+ and decrease in total phosphorylation state | Host gDNA degraded: 86%, starting at 1.5 h |
Light late | Late genes: high at 2.5 h, max at 4.5 h | ↑ PSII ↓ FNR, Cell div, Trx, Transl | Strong increase in NADPH/NADP+ and decrease in total phosphorylation state | Latent period: 8 h Burst size: ≥12 |
Dark early | Early genes: max at 30 min, then drop Middle genes: max at 1.5–2.5 h, then drop | Little response | Little change in infected or control | Host gDNA degraded: 44%, starting at 6.5 h |
Dark late | Late genes: max at 4.5 h, then drop | ↑ Transport, Trx, Transl ↓ FNR, Biosynth, PET, HLIPs, PSI | Total pool more oxidized and less phosphorylated in both infected and control | Latent period: N/A Burst size: N/A |
Events are listed for light and dark experiments, divided into early (0–2 h post-inoculation) and late (2–9 h post-inoculation) periods. Abbreviations: Biosynth, biosynthesis; Cell div, cell division; FNR, ferredoxin–NADP+ reductase; HLIPs, high light inducible proteins; PET, photosynthetic electron transport; PSII, photosystem II; PTOX, plastoquinol terminal oxidase; Transl, protein translation; Trx, transcription; N/A, not applicable.