Fig 2. Temperature effect on EGF photochemistry: A) EGF fluorescence excitation and emission wavelengths were fixed at 295 nm and 330 nm, respectively, at 10°C, 15°C, 20°C, 25°C and 30°C; B) SYPRO^® Orange fluorescence excitation and emission wavelengths were fixed at 470 nm and 580 nm, at the same temperatures.

Continuous illumination was conducted during 2 hours and the excitation slit size was set at 0.8 mm (1.67 μW) for all experiments.