Fig 9. SYPRO^® fluorescence excitation and emission spectra acquired before and after EGF 295 nm illumination for 2 hours.

SYPRO^® Orange fluorescence excitation and emission wavelengths were fixed at 470 nm and 580 nm, respectively. Excitation slit size was set at 2.0 mm (4.40 μW) and the temperature of each solution was kept at 20°C for all experiments.