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. 2016 Oct 27;11(10):e0165419. doi: 10.1371/journal.pone.0165419

Fig 14. EGFR binding assay in A549 cell model, for 1.5 hours in contact with treatment (100X).

Fig 14

CN1 corresponds to the non-treated cells, while CN2 shows the exposure to HAOA-coated gold nanoparticles (without any dye). As for the treatment groups: A1) free EGF with Alexa Fluor 647, B1) EGF-conjugated HAOA-coated gold nanoparticles (only EGF is marked with Alexa Fluor 647), and C1) EGF-conjugated HAOA-coated gold nanoparticles (both EGF and HAOA-coated gold nanoparticles are marked with Alexa Fluor 647 and Coumarin-6, respectively). For A2, B2 and C2, anti-EGFR antibodies were added 1 hour before the addition of the tested samples.