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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Mol Med (Berl). 2015 Dec 26;94(5):597–608. doi: 10.1007/s00109-015-1375-y

Figure 4. EPO-induced Stat5 phosphorylation in mtHEPOR fetal liver erythroid progenitors is augmented and sustained.

Figure 4

FLCs were obtained from mEpoR (M), wtHEPOR (wtH), and mtHEPOR (mtH) E14.5 fetuses and starved for 2 hours in RPMI + 10% FCS prior to stimulation with recombinant human (rh) EPO. The cell lysates (equivalent to 106 cells) were immunoblotted with a phopho-Stat5 (p-Stat5) antibody, followed by re-probing with antibodies specific for total Stat5 and GAPDH. A. EPO dose-response test of Stat5 activation. FLCs were stimulated with indicated concentrations of EPO for 15 minutes. B. Stat5 phosphorylation kinetic study. Analysis of p-Stat5 in FLCs stimulated with 1 U/ml of EPO for indicated periods of time. The relative quantification of p-Stat5 for mtHEPOR and mEpoR FLCs is presented graphically in Supplementary Fig. 1.