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. Author manuscript; available in PMC: 2017 May 8.
Published in final edited form as: J Mol Biol. 2016 Mar 22;428(9 Pt B):1833–1845. doi: 10.1016/j.jmb.2016.03.016

Figure 2.

Figure 2

Recent structures illuminate the architectures of gyrase and related type II topoisomerases. (a) 23 Å CryoEM map of the T. thermophilus gyrase holoenzyme in complex with 155 bp DNA, ciprofloxacin, and AMPPNP (reproduced from [21]). The domain architecture can be seen together with density attributable to DNA wrapped around the CTDs. Crystal structures of protein components and modeled DNA duplex (green) have been fit to the density. The closed N-gate is shown in a domain-swapped configuration first observed in (b) a crystal structure (reproduced from [22]) of the related enzyme S. cerevisiae topo II in complex with G-segment DNA (green) and AMPPNP. The DNA-gate and the C-gate are also seen in closed configurations in these structures.