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Characterization of proteolytic activity and ubiquitination in wild-type, R278C, and I79N mouse hearts. A, 26S proteasome activity of lysates of I79N, R278C, and wild-type mouse hearts (n = 3). B, 20S proteasome activity of lysates of wild-type, R278C, and I79N mouse hearts (n = 3). C, Western blot of free ubiquitin levels and ubiquinated proteins in transgenic mouse hearts (n = 3). D, Calpain activity (n = 3). *, p < 0.05. **, p < 0.001.
