Fig. 6.

Characterization of levels of oxidative stress-related proteins and antioxidant capacity in wild-type, R287C, and I79N mouse hearts. Western blot of enzymes associated with oxidative stress, A, Thioredoxin-1 (TRX-1) and B, Glutathione S-transferase mu 1 (GSTMI). C, Western blot of heat shock protein 60 (HSP60), which was not found to be differentially expressed by proteomic methods. D, Antioxidant capacity of cardiac homogenates were carried out using 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)/metmyoglobin. Antioxidant activity is measured relative to the antioxidant Trolox and reported in Trolox Equivalent Units (n = 4). *, p < 0.05.