Skip to main content
. 2016 Apr 20;15(6):2186–2202. doi: 10.1074/mcp.M115.057117

Fig. 1.

Fig. 1.

Scheme for the tagless fractionation. Ten grams of soluble protein cellular extract was subject to Ammonium Sulfate (AS) precipitation. Two out of the resulting six fractions were then subject to MonoQ ion exchange (Q-IEC) chromatography. 26 fractions from the Q-IEC column from the 38–48% AS step were separated by Hydrophobic interaction chromatography (HIC), whereas only 3 Q-IEC fractions from the 57–63% AS step were separated by HIC. 332 fractions from the HIC dimension were then each subject to Size exclusion chromatography (SEC), generating a set of 5273 SEC fractions that were subject to two dimensional iTRAQ mass spectrometry as described in Fig. 2A. Only a small subset of the HIC and SEC columns run are shown. The black lines below each fractionation step show those fractions subject to further separation or, in the case of the SEC fractions, to iTRAQ MS/MS analysis.