Fig. 2.

Global profiling of estrogen-regulated proteins as measured by SILAC. A, Statistical analysis revealed 13 and 96 proteins differentially expressed upon estradiol stimulation at 6 and 36 h of stimulation, respectively (fold changes >1.50 or <0.67 and FDR-adjusted t test p values <0.05 for 3 biological replicates). Identified proteins are shown as red dots, and fold change and p value cut-offs are presented as vertical and horizontal lines, respectively. See supplemental Table S1 for the full list of estrogen-regulated proteins. B, The search for molecular function and sub-cellular localization using the NextProt database (www.nextprot.org) revealed that the majority of identified proteins were involved in cell proliferation, cell cycle regulation, transcriptional regulation, nucleotide metabolism and DNA replication.